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Ramachandra, Y. L.
- Effect of Agrobacterium Infection Time, Co-Cultivation and Cell Density on in vitro Response in Hypocotyl of Eggplant (Solanum melongena L.)
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Authors
Affiliations
1 College of Horticulture, University of Horticultural Sciences, Munirabad - 583 233, Koppal, Karnataka, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga - 577 451, IN
3 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560 089, IN
1 College of Horticulture, University of Horticultural Sciences, Munirabad - 583 233, Koppal, Karnataka, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga - 577 451, IN
3 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560 089, IN
Source
Journal of Horticultural Sciences, Vol 11, No 1 (2016), Pagination: 21-26Abstract
The present study purports to assess the effect of Agrobacterium infection time, co-cultivation and cell density on in vitro response in hypocotyl explants of eggplant (brinjal) cv. Manjarigota. Agrobacterium (OD600 0.3-0.5) infection for 10-15 minutes (24.44±2.34%) was found to be optimum, while, higher or lower infection-time resulted in reduced callus initiation, shoot regeneration and explant survival. Explants with no (only Agrobacterium infection) or short (1 day) co-cultivation, showed reduced callus-initiation response and turned yellow, with no regeneration. Callus-initiation response increased from Day 1 (96.66±03.33%), and reached a maximum on Day 2 and Day 3 (100±00.00%). It decreased on further increase in co-cultivation time. Explants co-cultivated for three days showed highest regeneration response (30.00±02.96%) which thereafter reduced with further increase in co-cultivation time. Explants infected with Agrobacterium culture at 0.05 OD600 showed hardly any regeneration, and turned yellow and necrotic on the selection medium. Highest regeneration response (28.33±02.33%) was obtained in explants infected with 0.1 OD600 culture, and this gradually reduced as celldensity increased (upto 1.0 OD600), becoming zero in explants treated with cultures at 1.5 OD600 or above. Agrobacterium overgrowth was noticed on explants infected with cultures of 0.5 OD600 and above. Exposure of hypocotyl explants to higher cell-density, longer infection-time and prolonged co-cultivation regime resulted in severe necrosis of explants; time taken for development of Agrobacterium overgrowth was less with increase in the level of these factors. Regenerated shoots were healthy, green, elongated and showed ischolar_main induction on culture medium containing Kanamycin.Keywords
Eggplant, Manjarigota, Regeneration, Agrobacterium, PCR.References
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- Hanur, V.S., Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Riaz Mahmood, and Lalitha Anand. 2006. Synergistic use of hypocotyl explants and high BAP pre-conditioning for enhanced transformation frequency in brinjal (Solanum melongena L.). J. Hortl. Sci., 1:116-119
- Kumar, V.S. and Rajam, M.V. 2005. Enhanced induction of vir genes results in the improvement of Agrobacterium-mediated transformation of eggplant. J. Biochem. Biotech., 14:89-94
- Magioli, C. and Mansur, E. 2005. Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant. Acta Bot. Bras., 19:139-148
- Magioli, C., Rocha, A.P.M., Pinheiro, M.M., Martins, S.G. and Mansur, E. 2000. Establishment of an efficient Agrobacterium-mediated transformation system for eggplant and study of a potential biotechnologically useful promoter. J. Pl. Biotech., 2:43-49
- Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tissue cultures. Physiol. Plantarum, 15:473-497
- Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Lalitha Anand and Vageeshbabu S. Hanur. 2007a. Effect of explant type and growth regulators in the transformation of brinjal (Solanum melongena L.) cv. Manjarigota. J. Hortl. Sci., 2:94-98
- Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Lalitha Anand and Vageeshbabu S. Hanur. 2007b. Effect of antibiotics and gelling agents in the transformation of brinjal (Solanum melongena L.) cv. Manjarigota. J. Hortl. Sci. 2:19-25
- Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Shetti, D.L., Lalitha Anand and Vageeshbabu S. Hanur. 2008. Effect of growth regulators on in vitro complete plant regeneration in brinjal (Solanum melongena L.). Ind. J. Hort., 6:112-116
- Veluthambi, K., Gupta, K.A. and Sharma, A. 2003. The current status of plant transformation technologies. Curr. Sci., 84:368-379
- Zhang, Z., Coyne, D.P. and Mitra, A. 1997. Factors affecting Agrobacterium-mediated transformation of common bean. J. Amer. Soc. Hortl. Sci., 122:300-305
- Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
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PDF Views:117
Authors
Affiliations
1 College of Horticulture, Munirabad-583 233, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga-577 451, IN
3 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560 089, IN
1 College of Horticulture, Munirabad-583 233, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga-577 451, IN
3 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560 089, IN
Source
Journal of Horticultural Sciences, Vol 10, No 2 (2015), Pagination: 136-142Abstract
An attempt was made to assess the effect of size, age and position of the explant, pre-culture and high cytokinin concentration in the pre-culture medium on shoot regeneration in brinjal hypocotyls co-cultivated with Agrobacterium. The study was carried out using hypocotyl explants of brinjal cv. Manjarigota, Agrobacterium strain A208 and shoot regeneration medium (full-strength basal MS medium, 2μM BAP + 0.05μM NAA, 3% sucrose and 0.8% agar) containing Cefotaxime (250-500mg l-1) and Kanamycin (100mg l-1). Hypocotyl explants showed callus initiation and shoot regeneration response after 10-12 and 20-22 days of culture, respectively. Five-day-old explants did not survive Agrobacterium infection, and ten-day-old explants showed higher shoot regeneration (29±1.91%) than older explants. Explants of medium size (1cm long; 32±2.62%) from the apical region (38.57±2.61%) showed better shootregeneration ability than explants of any other size or region. A period of four days of pre-culture (33.33±3.76) was optimal best for best shoot-regeneration in hypocotyl explants. No regeneration was seen in hypocotyl explants at shorter or longer pre-culture period. High cytokinin (10μM) in shoot regeneration medium during pre-culture enhanced shoot regeneration response (47.27±2.98%) in explants co-cultivated with Agrobacterium. Effects of various factors documented in this study will be useful in developing an efficient Agrobacterium-mediated transformation protocol in brinjal cv. Manjarigota.Keywords
Eggplant, Pre-Culture on High BAP, Explant Characters, PCR.References
- Balappa, S.R. and Hugar, L.B. 2002. An economic evaluation of brinjal production and its marketing system in Karnataka. Agril. Market, 44:45-49
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- Chen, Q., Jelenkovic, G., Chin, C., Billings, S., Eberhardt, J. and Goffreda, J.C. 1995. Transfer and transcriptional expression of coleopteran Cry3B endotoxin gene of Bacillus thuringiensis in eggplant. J. Amer. Soc. Hort’l. Sci., 120:921-927
- Chy, Y.S. and Philips, G.C. 1987. High efficiency Agrobacterium-mediated transformation of Lycopersicon based on conditions favaourable for regeneration. Pl. Cell Rep., 6:105-108
- Collonier, C., Fock, I., Kashyap, V., Rotino, G.L., Daunay, M.C., Lian, Y., Mariska, I.K., Rajam, M.V., Servaes, A., Ducreux, G. and Sihachakr, D. 2001. Applications of biotechnology in eggplant. Pl. Cell Tiss. Org. Cult., 65:91-107
- Compton, M.E. 2000. Interaction between explant size and cultivar affects shoot organogenic competence of watermelon cotyledons. Hort’l. sci., 35:749-750
- Curuk, S., Elman, C., Schlarman, E., Sagee, O., Shomer, I., Cetiner, S., Gray, D.J. and Gaba, V. 2002. A novel pathway for rapid shoot regeneration from the proximal zone of the hypocotyl of melon (Cucumis melo L.). In Vitro Cell. Dev. Biol.-Plant, 38:260-267
- Fari, M., Nagy, I., Csányi, M., Mityko, J. and Rásfalvy, A. 1995. Agrobacterium-mediated genetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (Solanum melongena L. cv. ‘Kecskemeti lila’). Pl. Cell Rep., 15:82-86
- Frary, A. and Earle, E.D. 1996. An examination of factors affecting the efficiency of Agrobacterium-mediated transformation of tomato. Pl. Cell Rep., 16:235-240
- George, E.F. 1993. Plant propagation by tissue culture. Part 1. The technology. Exegenetics Ltd., Eddington, England
- Hamza, S. and Chupeau, Y. 1993. Re-evaluation of conditions for plant regeneration and Agrobacterium-mediated transformation from tomato. J. Expt’l. Bot., 44:18371845
- Hanur, V.S., Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Riaz Mahmood and Lalitha Anand. 2006. Synergistic use of hypocotyl explants and high BAP pre-conditioning for enhanced transformation frequency in brinjal (Solanum melongena L.). J. Hort’l. Sci., 1:116-119
- Herath, P.S., Suzuki, T. and Hattori, K. 2005. Factors influencing Agrobacterium-mediated genetic transformation of Kenaf. Pl. Cell Tiss. Org. Cult., 82:201-206
- Kumar, V.S. and Rajam, M.V. 2005. Enhanced induction of vir genes results in the improvement of Agrobacterium-mediated transformation of eggplant. J. Biochem. Biotech., 14:89-94
- Lazzeri, P.A. and Dunwell, J.M. 1986. In vitro regeneration from seedling organs of Brassica oleracea var. italica Plenck. cv. Green Comet. II: Effect of light conditions and explant size. Annl. Bot., 58:699-710
- Ling, H., Kriseleit, D. and Ganal, M.W. 1998. Effect of ticarcillin/ potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato. Pl. Cell Rep., 17:843-847
- Magioli, C. and Mansur, E. 2005. Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant. Acta Bot. Brassilica, 19:139-148
- Magioli, C., Rocha, A.P.M., Pinheiro, M.M., Martins, S.G. and Mansur, E. 2000. Establishment of an efficient Agrobacterium-mediated transformation system for eggplant and study of a potential biotechnologically useful promoter. J. Pl. Biotech., 2:43-49
- Murashige, T. and Skoog, F. 1962. A revised method for rapid growth and bioassays with tissue cultures. Physiol. Pl., 15:473-497
- Nehra, N.S., Chibbar, R.N., Kartha, K.K., Datla, R.S.S., Crosby, W.L. and Stushnoff, C. 1990. Agrobacterium-mediated transformation of strawberry calli and recovery of transgenic plants. Pl. Cell Rep., 9:10-13
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- Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Lalitha Anand and Vageeshbabu S. Hanur. 2007a. Effect of explant type and growth regulators in the transformation of brinjal (Solanum melongena L.) cv. Manjarigota. J. Hort’l. Sci., 2:94-98
- Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Lalitha Anand and Vageeshbabu S. Hanur. 2007b. Effect of antibiotics and gelling agents in the transformation of brinjal (Solanum melongena L.) cv. Manjarigota. J. Hort’l. Sci., 2:19-25
- Prakash, D.P., Deepali, B.S., Asokan, R., Ramachandra, Y.L., Shetti, D.L., Lalitha Anand and Vageeshbabu S. Hanur. 2008. Effect of growth regulators on in vitro complete plant regeneration in brinjal (Solanum melongena L.). Ind. J. Hort., 64:112-116
- Sanyal, I., Singh, A.K., Kaushik, M. and Amla, D.V. 2005. Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) with Bacillus thuringiensis cry1Ac gene for resistance against pod borer insect Helicoverpa armigera. Pl. Sci., 168:1135-1146
- Sharma, P. and Rajam, M.V. 1995. Genotype, explant and position effects on organogenesis and embryogenesis in eggplant (Solanum melongena L.). J. Expt’l. Bot., 46:135-141
- Veluthambi, K., Gupta, K.A. and Sharma, A. 2003. The current status of plant transformation technologies. Curr. Sci., 84:368-379
- Effect of Age and Size of Hypocotyl Explant on in vitro Shoot Regeneration in Eggplant
Abstract Views :159 |
PDF Views:117
Authors
Affiliations
1 College of Horticulture, Munirabad-583 233, Koppal, UHS Bagalkot, Karnataka, IN
2 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore 560 089, IN
3 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga 577 451, IN
1 College of Horticulture, Munirabad-583 233, Koppal, UHS Bagalkot, Karnataka, IN
2 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore 560 089, IN
3 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga 577 451, IN
Source
Journal of Horticultural Sciences, Vol 7, No 2 (2012), Pagination: 203-205Abstract
In the present study, effect of size and age of hypocotyl explant on in vitro organogenetic responses was assessed in eggplant cv. Manjarigota. Size and age did not affect callus-initiation response, but showed marked influence on shoot regeneration response. Hypocotyl explants 1.5cm long showed highest shoot regeneration response (77.4%); either increase or decrease in size resulted in reduced response. Five to 15 day old hypocotyl explants showed direct shoot regeneration from cut ends, whereas 20-30 day old hypocotyl explants showed indirect shoot regeneration from callus produced on cut ends. Five day old explants were most responsive, with highest (91.23%) and thirty day old explants least responsive with reference to shoot regeneration response (20.85%). Shoot regeneration frequency decreased with increasing age, whereas shoot regeneration efficiency increased with increasing age of hypocotyl explants.Keywords
Eggplant, Shoot Regeneration, Age and Size, Hypocotyl Explants.- Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
Abstract Views :209 |
PDF Views:110
Authors
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Lalitha Anand
1,
Vageeshbabu S. Hanur
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 2, No 2 (2007), Pagination: 94-98Abstract
Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.Keywords
Growth Regulators, Explant, Brinjal, Transformation.- Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
Abstract Views :227 |
PDF Views:114
Authors
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Lalitha Anand
1,
Vageeshbabu S. Hanur
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 2, No 1 (2007), Pagination: 19-25Abstract
A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that showed increased callus initiation and regeneration response upon cocultivation with Agrobacterium and on kanamycin at 100 mg/l were selected as this indicated a highly effective selection pressure. Cefotaxime did not affect regeneration response and at 500 mg/l, it effectively inhibited Agrobacterium overgrowth completely on Agrobacterium cocultivated hypocotyl explants. There were marked differences in regeneration response in hypocotyl explants cultured on medium solidified with various gelling agents indicating the influence of gelling agent on the activity of kanamycin in culture medium, which indirectly affects selection and recovery of transformants. Antibiotics and gelling agents could therefore affect, directly or indirectly, transformation of brinjal cv. Manjarigota.Keywords
Solanum melongena, Kanamycin, Cefotaxime, Gelling Agents, Transformation.- Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
Abstract Views :216 |
PDF Views:113
Authors
Vageeshbabu S. Hanur
1,
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Riaz Mahmood
2,
Lalitha Anand
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 1, No 2 (2006), Pagination: 116-119Abstract
Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal after Agrobacterium infection to the explants. The effect of explant was studied and hypocotyls were found to be better than cotyledonary leaves. High BAP during the preconditioning period was found to further enhance the regeneration rate. Therefore, use of hypocotyls and high BAP during preconditioning can improve the regeneration of transformed cells and recovery of transformants in vegetables especially brinjal.Keywords
Solanum melongena, Transformation, Hypocotyl, BAP, Preconditioning.- Phytopharmacological Evaluation of Leaf Extracts of Hemidesmus indicus (L)
Abstract Views :212 |
PDF Views:0
Authors
Affiliations
1 Department of Biotechnology and Bioinformatics, Kuvempu University, Shimoga (Karnataka), IN
1 Department of Biotechnology and Bioinformatics, Kuvempu University, Shimoga (Karnataka), IN
Source
Asian Journal of Bio Science, Vol 3, No 2 (2008), Pagination: 237-239Abstract
For centuries, indigenous medicinal plants have been used against bacterial-induced pathogenesis. Hemidesmus indicus is a pharmacologically important plant. The in vitro experiment by the agar well diffusion assay showed the presence of bioactive components in H. indicus leaf extract through zone of inhibition. In different ratios of petroleum ether and ethyl acetate test have shown significant zone of inhibition against gram positive and gram negative bacteria: Bacillus subtilis (54 mm), Escherichia coli (70 mm) and Pseudomonas aeruginosa (50 mm).Keywords
Antibacterial Activity, H. indicus, Medicinal Plants, Bioactive Components.- Evaluation of Relative Moisture Loss from Leaves of Five Mulberry Varieties During Silkworm Rearing
Abstract Views :182 |
PDF Views:0
Authors
Y. L. Ramachandra
1,
S. Padmalatha Rai
2,
H. V. Sudeep
1,
P. S. Sujan Ganapathy
1,
B. T. Kavitha
1,
N. B. Krishnamurthy
3
Affiliations
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Centre, Manipal University, Manipal (Karnataka), IN
3 Department of Biotechnology, Shridevi Institute of Engineering and Technology, Tumkur (Karnataka), IN
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Centre, Manipal University, Manipal (Karnataka), IN
3 Department of Biotechnology, Shridevi Institute of Engineering and Technology, Tumkur (Karnataka), IN
Source
Asian Journal of Bio Science, Vol 3, No 2 (2008), Pagination: 245-246Abstract
Leaf moisture content and moisture retention rate are important qualitative parameters from the point of young age silkworm rearing. In the present investigation relative moisture loss from tender leaves of five selected mulberry varieties viz. S36, S54, DD, V1 and M5 from Shimoga district were evaluated. Differences among the five varieties with respect to the leaf moisture retention capacity/moisture loss are included in this study. Based on the values recorded, variety V1 showed higher retention rate at different hours of preservation compared to rest of the varieties. The lowest rate was recorded from M5 variety.Keywords
Leaf Moisture Loss, Retention Rate, Mulberry Varieties.- Evaluation of Soil Fertility and Mulberry Leaf Quality on Silkworm Rearing and Cocoon Characteristics
Abstract Views :179 |
PDF Views:0
Authors
Y. L. Ramachandra
1,
S. Padmalatha Rai
2,
H. V. Sudeep
1,
P. S. Sujan Ganapathy
1,
N. B. Krishnamurthy
3
Affiliations
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Center, Manipal University, Manipal (Karnataka), IN
3 Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur (Karnataka), IN
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Center, Manipal University, Manipal (Karnataka), IN
3 Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur (Karnataka), IN
Source
Asian Journal of Bio Science, Vol 3, No 2 (2008), Pagination: 295-300Abstract
Soil samples were collected from six different mulberry growing areas of Malnad region of Karnataka and analyzed for the soil fertility and leaf quality parameters. The influence of soil nutrient status on mulberry plant growth and leaf quality with regard to five varieties M5, DD, S54, S36 and V1 and the overall impact on silkworm larval growth and cocoon characteristics were studied in detail. It was observed that the six sampling areas differed significantly in the parameters analyzed. Variety V1 from Kadur region showed the best result among all the five varieties grown in six sampling areas.Keywords
Cocoon Characteristics, Leaf Quality, Mulberry Growth, Larval Growth, Soil Nutrient Status.- Antibacterial Activity of Correa Leaves Extract
Abstract Views :221 |
PDF Views:0
Authors
C. C. Gavimath
1,
Y. L. Ramachandra
1,
S. Padmalatha Rai
2,
H. V. Sudeep
1,
P. S. Sujan Ganapathy
1,
B. T. Kavitha
1
Affiliations
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Center, Manipal University, Manipal (Karnataka), IN
1 Department of Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Jnana Sahyadri, Shimoga (Karnataka), IN
2 Department of Biotechnology, Manipal Life Science Center, Manipal University, Manipal (Karnataka), IN